On 27 Oct 1755 when Samuel was 40, he married Mary Mayo (24872) , daughter of Judah Mayo (9701) (25 Sep 1691-ca 1761) & Mary Hamilton (40047) (6 Aug 1693-), in Trinity Church, Newport. Born ca 1725. Mary died in Port Medway, Nova Scotia on 1 Oct 1811.

After Jospeh’s death, Mary married a Mr. Freeman. On 2 Dec 1761 Thomas Hamilton was appointed guardian of Hezekiah and Mary, the two surviving children.254