On 4 May 1865 Jan married Jantina Lingbeek, daughter of Fokke Jans Lingbeek (12 Aug 1809-29 May 1877) & Hindriktje Jacobs Lingbeek (31 Jul 1802-16 Dec 1891), in Nieuwe Pekela, Groningen.538 Born ca 1839 in Nieuwe Pekela, Groningen. Jantina died in Nieuwe Pekela, Groningen on 19 Nov 1911.538

Children of Jan Twiest and Jantina Lingbeek, born at Nieuwe Pekela, include:
    i. Hinderkien Twiest, b. 1867, d. 11 Feb 1929 at Gasselte, Drenthe, m. at Nieuwe Pekela 10 May 1893 Jan Nijhof (b. ca. 1868 at Noorddijk, Groningen, son of Hielke Nijhof & Hinderkien Redeker;
    ii. Menso Twiest, b. 1869, m. at Nieuwe Pekela 6 Jun 1901 Katharina van Dijk (b. 1874 at Nieuwe Pekela, dau. of Klaas van Dijk & Elsien Piebes);
    iii. Fokko Twiest, b. ca. 1872, d. 12 Dec 1901 at Nieuwe Pekela; and
    iv. Houwkina Twiest, b. 1877, m. at Nieuwe Pekela 15 May 1901 Fokko Niewold (b. 1866 at Nieuwe Pekela, son of Tammo Niewold & Jantje Poortman).